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BCECF-AM
Ageladine A
LysoGlow84
DMSP

LysoGlow84


Intrazellular pH-Indikator


Properties


- No esterases involved, therefore no toxic byproducts

- Wide pH range of fluorescence  (pH 3 to pH 13)

- Long-term stable; barely metabolized

- For acidic organelles, cells, tissue and small transparent whole animals even in long-term    experiments over several days

- Ratiometric evaluation of pH (F400/440)

- Highly pure, stable and untoxic



Based on the known chemical structure and chemical synthesis of the sponge alkaloid Ageladine A we synthesized the Ageladine A-derivative LysoGlow84. Structure and purity of the synthesized LysoGlow84 was confirmed by NMR spectroscopy and mass spectrometry. The fluorescence intensity emitted by LysoGlow84 depended strongly on the pH of the environment with highest fluorescence intensity recorded between pH4 and pH6 in the blue to green color range. The fluorescence maximum (330 nm excitation) was found at 440 nm.

Exposure of cultured rat brain astrocytes for hours to micromolar concentrations of LysoGlow84 did not compromise the cell viability as demonstrated by several viability assays. However, application of LysoGlow84 to astrocytes revealed promising properties of the compound to stain lysosomes. Conventional fluorescence microscopy (Leica and Zeiss) of LysoGlow84-treated astrocytes revealed a dotted cellular staining pattern and an almost perfect co-localization of LysoGlow84 fluorescence with that of Lysotracker Red®. By confocal scanning microscopy (Leica SP5) large overlaps in cellular fluorescence and only small differences in stained structures were observed, if the cells had been stained with both dyes. Thus, LysoGlow84 is a new promising dye that stains lysosomes in viable mammalian cells. Additionally, specific structures in the transparent marine flatworm Macrostomum lignano are well stained, which are not marked by the Lysotracker Red®.

Conclusion: Large overlaps in cellular fluorescence and small differences in stained structures were observed when the cells had been stained with LysoGlow8 4 and LysoTracker Red. In marine worms larger differences in stained organs are observable. LysoGlow84 is a new promising dye that stains lysosomes in viable mammalian cells and specific organs in marine flatworms.





Innovations from the Sea

Downloads

Data sheet LysoGlow84 (pdf)

MSDS LysoGlow84 (pdf)